T Vector for Direct Selection Using Green Fluorescent Protein

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T vector for direct selection using green fluorescent protein.

fied tubulins, p. 478-485. In R.B. Vallee (Ed.), Methods in Enzymology, Vol. 196. Academic Press, New York. 3.Nogales, E., S.G. Wolf and K.H. Downing. 1998. Structure of the αβ tubulin dimer by electron crystallography. Nature 391:199-203. 4.Schiff, P.B., J. Fant and S.B. Horwitz. 1979. Promotion of microtubule assembly in vitro by taxol. Nature 277:665-667. 5.Schiff, P.B. and S.B. Horwitz. 198...

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Positive-selection vector for direct protein expression.

We describe the development of a novel positive-selection vector, RHP-AmpS, that is suitable for seamless cloning and high-level protein expression in Escherichia coli. In this vector, beta-lactamase (Bla) was rendered nonfunctional by replacing the codon for the C-terminal amino acid of the beta-lactamase gene (bla) with a stop codon. Insertion of a target gene in the correct orientation (tail...

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Fluorescent protein vector for directional selection of PCR clones.

Green fluorescent protein (GFP) has become a convenient and versatile tool as a reporter protein in many aspects of science. Here, we show that the enhanced yellow fluorescent protein (EYFP) variant may be used advantageously as a reporter system for directional cloning of blunt-ended PCR products. We have constructed a pUC18-derived plasmid containing a reporter gene coding EYFP cloned into th...

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ژورنال

عنوان ژورنال: BioTechniques

سال: 1998

ISSN: 0736-6205,1940-9818

DOI: 10.2144/98252bm03